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Most people who start smoking do so before the age of 9 and are easily addicted, many for life

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Washed cells were resuspended in 5 ml complete RPMI tissue culture media supplemented with 10% FCS, underlaid with 4 ml of room temperature Ficoll-Paque (GE Healthcare), and centrifuged for 20 min, 1400 g , room temperature, brake off