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Overall, determining a level of harm reduction for all ENDS devices is extremely difficult, as some open-system third-generation ENDS devices emit carbonyls at levels orders of magnitude higher than those emitted from first, second or fourth-generation ENDS devices 126-130

The only solvent used in the process are natural terpenes, which are food grade and made for human consumption

To accomplish this, reagents and working standards were prepared according to the instructions provided by the ELISA kit (D6050, DTA00D, R&D systems, USA) and kept the room temperature for 20 min

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