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To assess whether R2-mediated TPRT could support the integration of a full-length gene cassette, we designed a donor RNA template encoding a functional gene circuit comprising a minimal synthetic promoter (pMinSyn), a GFP ORF (GFinP) and the nos terminator (NOSter), guided by previously established mammalian reporter systems 68,69

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Since the current study focused on a relatively younger and male population, age-related morphological alterations in the gingival tissues may become more apparent in older age, specifically after the 4th decade 37,38

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