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According to previously a published method 10 , protocol and MTT assay, C646 (Topscience, T2452, Shanghai, China) was prepared in 12.5 mM solution, and CTPB (Abcam, ab142224, London, UK) was prepared in 25 mM solution

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Smokers also need to be informed prior to the commencement of periodontal treatment that the outcome of such treatment may be compromised if they continue to smoke

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