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The unlabeled fluorophores were separated from the labeled protein solution using a resin column (provided in the kit) equilibrated previously with 1 PBS elution buffer (0.1 M potassium phosphate, 1.5 M NaCl, pH 7.2, with 2 mM sodium azide)

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The clinics and the hospital serve local residents, tourists, and sailors from the hundreds of cruising sailboats that visit the sheltered harbor each year, providing antibiotics, drugs to treat diabetes, pain medication, as well as other drugs as needed

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