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The supernatant was then decanted into a prelabelled sample vial, and after a 5 min equilibration period, pH measurements were performed in accordance with the ISO 17025 validated method.25 26 Measurement of nicotine Total nicotine concentrations were measured using a gas chromatography-mass spectrometry method in selected ion monitoring mode (SIM), previously described, with slight modifications to the extraction process.25 27 Lozenges, chewing gum and toothpicks were cut using a scissor into pieces as necessary

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Normal findings are not expected

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