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Statistical analysis For biochemical analysis, summary statistics were analysed using SPSS 20.0 and the mean standard deviation (SD) were tabulated in Tables 1, 2, 3

Determination of Mainstream Tobacco Smoke pH Health Canada T-113 December 31, 1999 Table of Contents Scope of Applications Normative references Definitions Method Summary Apparatus and Equipment Reagents and Supplies Preparation of Glassware Preparation of Solutions Preparation of Standards Sampling Tobacco Product Preparation Smoking Machine, Electrode and pH Meter Preparation Sample Generation Sample Analysis Quality Control Modifications for Intense Smoking References Appendices 1 Scope of Applications This method is to be used to determine the pH of mainstream tobacco smoke on a puff-by-puff basis in order to observe the acid-base effects of mainstream smoke on an aqueous film during the smoking process

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While normally tobacco is air-cured, these leaves are left to hang in specially-constructed curing barns