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Extraction of salivary DNA and next-generation sequencing Saliva aliquots of 600 l were shipped to CoreBiome (OraSure, Bethlehem, PA, USA) where they were extracted with MO Bio PowerFecal (Qiagen, Hilden, Germany) automated for high throughput on QiaCube (Qiagen), with bead-beating in 0.1 mm glass bead plates