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Western blotting Cells were lysed in a protein lysis buffer, and protein concentrations were determined by Bradford assays

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Assessment of model performance The modelled annual population prevalence (modelled using smoking initiation and cessation rates, and adjusted for the increased mortality risk of smokers) of current and former smokers was compared with historic survey results using the Survey of Smoking Habits (1971 to 1986), Note 11 Note 14 Note 15 Note 16 Note 17 Note 18 Note 19 Note 20 Note 21 Note 22 the Canadian Health Survey (1978), Note 23 the General Social Survey (1985 and 1991), Note 16 Note 17 the Ontario Health Survey (1990), Note 18 and the National Population Health Survey (1994 to 1998)

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