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After a 2-day treatment with acrolein, the cells were lysed using Passive Lysis Buffer (Promega, USA) for 30 min at 37 C

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2 David Harley, The Beginnings of the Tobacco Controversy, Bulletin of the History of Medicine , Spring 1993, p

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One short arc of Bloom County had Binkley's father going through withdrawal when Binkley hides his cigarettes