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Spliffs: cannabis mixed with tobacco in rolling paper spliff smokers blend the two directly in the filler, with personal preference determining the cannabis-to-tobacco ratio

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Additional information Peer review information Nature Reviews Cancer thanks David Ashley and the other, anonymous, reviewer(s) for their contribution to the peer review of this work

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However, it cannot be excluded that except for nicotine, chemicals like acrolein and methyl vinyl ketone, present in TS and e-cigs have been shown to cause oxidative stress by the generation of ROS through activation of protein kinase C via NADPH oxidase (Asano et al., 2012)