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in PI uptake assay, the number of the cells positive for PI uptake was represented as a percentage of total number of cells identified by Hoechst 33342 for nuclear staining

Theres also the fact that while theyre not nearly as common as they used to be, Japan has sold alcoholic beverages in vending machines, with no ID check required, for generations

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Du H, Wang X, Zhang Y, Xu Y (2019) Exploring the impacts of raw materials and environments on the microbiota in Chinese Daqu starter