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The specific steps were as follows: First, BAM files generated from the mapping of tobacco leaf samples to the reference genome were sorted using SAMtools (v1.21) ( Subsequently, the SplitNCigarReads function in GATKwas applied to split reads into exon segments (removing Ns while maintaining grouping information) and hard-clip any sequences overhanging into intronic regions ( Identification of sQTL ASEs were identified using LeafCutter ( Results Extensive transcriptome diversity and complexity in tobacco In this study, a total of 207,689 transcripts were identified from 599 transcriptome datasets derived from 13 different tissues

In 2008, the World Health Organization named tobacco use as the world's single greatest preventable cause of death

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Individualism: A man defined by his choices, not societys expectations

Tokyo-based Japan Tobacco, of which the countrys government owns 37%, controls 60% of the local cigarette market

Born in 1884, Dr