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Evaluating cotinine levels in urine, saliva, and plasma is an effective method to gauge tobacco exposure, as cotinine in an unstimulated total salivary flow rate has a relatively long half-life [ The current findings indicated that UCot levels were significantly higher among smokers (490.19 560.34 ng/mL), e-cig users (175.87 315.58 ng/mL), and dual smokers (559.74 588.53 ng/mL) compared to non-smokers (133.70 266.34 ng/mL)

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