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Concentrations were based on previous studies, and cells were incubated at 37C with 5% CO2 for 4 h ( CD4 + T cells were plated at a density of 1 10 6 cells/well in 24-well plates in 1 ml RPMI 1640 complete medium containing fumaric acid (173 M) or dimethyl sulfoxide (DMSO,ST038, BYT, Shanghai, China) as a vehicle control

Equilibrium Constants of Exchange Reactions of Cystine with Glutathione and with Thioglycolic Acid Both in the Oxidized and Reduced State

and a combination of 0.5 M rotenone and antimycin A, which inhibits the electron transport chain by blocking complex I and III, respectively

Nature 517 , 293301 (2015)