Zhongxiang Wang et al
However, excluding the chemolytic or selective chemical recycling techniques for polyesters and similar plastics 5,6,7,8 , chemical recycling to monomer of commercial vinyl plastics currently relies upon thermolysis, where high temperatures in the absence of oxygen are used to homolytically break polymer bonds 9
pGEX series expression plasmid Plasmid size: 4969bp Plasmid label: N-GST, N-thrombin Prokaryotic resistance: ampicillin Amp Cloned strain: DH5 alpha Culture conditions: 37 centigrade, aerobic, LB Expression host: Escherichia coli Inducement: IPTG or lactose and its analogues 5'sequencing primers: pGEX5: GGGCTGGCAAGCCACGTTTGGTG 3'sequencing primers: pGEX3: CCGGGAGCTGCATGTGTCAGAGG Note: the recombinant protein can be purified by GST affinity column Use:pGEX Plasmid pGEX-4T-1 Description PGEX-4T-1 is a E.coli expression plasmid pGEX-4T-1 Sequence LOCUS Exported 4969 bp ds-DNA circular SYN 03-8-2015 DEFINITION synthetic circular DNA KEYWORDS pGEX-4T-1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4969) TITLE Direct Submission JOURNAL Exported 2015-8-3 FEATURES Location/Qualifiers source 1..4969 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 183..211 /note="tac promoter" /note="strong E
Franklin CC, Backos DS, Mohar I, White CC, Forman HJ, et al
Cognitive and psychological problems such as memory loss, confusion, irritability, or depression appear after prolonged deficiency of vitamin B12
Annals of Clinical Biochemistry, 43(5), 344- 353
