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The RAW files were uploaded to Integrated Proteomics Pipeline (IP2) and searched using the ProLuCID algorithm (publicly available at using a reverse concatenated, non-redundant version of the Mouse UniProt database (release 2017)

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Synthetic application To further explore the mild nature of our method, an HBT-substrate 1v was subjected to the reaction condition in a mixed solvent of 95% PBS buffer and 5% 1,4-dioxane (Fig

1H), suggesting conserved function among GPX paralogs