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Cell 170 , 10621078 (2017)

Artesunate inhibits TGF-1-induced epithelial-to-mesenchymal differentiation (EMT) and RLE-6TN cell proliferation by upregulating Smad7 mRNA and protein expressions and downregulating Smad3, ACTA2, and Vim mRNA expressions ( 5.2.4 Promotes iron-mediated autophagy Wang et al

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The plasma supernatant was transferred and the remaining cell pellet was diluted with 2.6 mL of 0.1% Triton X-100 and 0.6% sulfosalicylic acid in KPE (0.1 M potassium phosphate buffer with 5 mM EDTA disodium salt, pH 7.5) for homogenization