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Researchers working with this assay commonly use metaphosphoric acid (MPA) or perchloric acid (PCA) to deproteinize samples immediately upon collection

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For the third and subsequent time points, repeat step 6 for an additional 5 minute-interval until the completion of the kinetics

36 In patients with celiac disease, along with formation of IgA antibodies to tissue transglutaminase, there is also formation of IgA antibodies to epidermal transglutaminase