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TMAO and Atherosclerotic Thrombosis In animal models, TMAO has been shown to directly cause AS and thrombosis (Koeth et al., 2013

The mobile phase consisted of A: 60:40 acetonitrile:water (v/v) and B: 90:10 isoproanol:acetonitrile (v/v), both with 10 mM ammonium formate, 0.1% formic acid and 0.1 M reserpine (lock mass)