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Methods to prevent excessive oxidative harm to healthy tissues including targeted administration of pro-oxidant agents, antibodydrug conjugates, and prodrugs activated in tumour -specific environments [237]

doi: 10.1007/s10571-023-01388-8 36 WangBWangYZhangJHuCJiangJLiYet al

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Immunohistochemistry analysis Mice in different treatment groups were anesthetized with pentobarbital sodium (Sigma-Aldrich, P-010) and perfused with PBS followed by 4% paraformaldehyde in PBS

Oocyte ATP levels The ATP level of oocytes was determined as luminescence emitted in an ATP-dependent luciferinluciferase bioluminescence assay (ATP assay kit