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Biol Psychiatry (2012) 71:60310.10.1016/j.biopsych.2011.10.038 138 LiuJBlondBNvanDyckLISpencerLWangFBlumbergHP

Here's the most important part of the technique: Aim the needle at the inside glass wall of the vial

Reads were aligned and annotated to the Ensembl Homo sapiens GRC38.p13 reference genome and associated GTF annotation file using the align and featureCounts functions from the Rsubread package (2.10.4) in R (4.2.0)

2011;343:d6407