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coli ET08, were constructed by deleting genes of pta , ldhA , and pykF in E

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We notice that by increasing the amount of (CENP-A/H4) 2 relative to DNA, we enrich the faster traveling particle, so we concluded that the particle corresponding to the slower band on gel is a tetrasome, whereas the one that travels faster and almost the same as the CENP-A nucleosome is the 2x(CENP-A/H4) 2 octasome (Fig S1A and C)