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Description

Experiments with [5- 13 C] glutamine Cells were incubated in 2-well Lab-Tek chambers for 2022 h in either standard cell culture media supplemented with 10% FBS and penicillin (50 U/mL)-streptomycin (10 g/mL) or glutamine-free RPMI-1640 media (Corning, VA) supplemented with 1.5 mM [5- 13 C] glutamine, 10% FBS, and penicillin (50 U/mL)-streptomycin (10 g/mL) at 37C/5% CO 2