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doi: 10.1038/s41580-020-0230-3 11 MurphyMP

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The samples, after reaction with modificators were purified two times using Zeba Spin Desalting Columns 7 K MWCO (ThermoFischer Scientific)

Similarly, solid lipid nanoparticles loaded with curcumin and turmerone were optimized using response surface methodology for topical delivery, resulting in enhanced stability, controlled release, and reduced irritation [149]

Specifically, ARPE19 cells (150 cm 2 ) were cultured as described above until 80% confluency, harvested, washed with PBS, and resuspended in 7 mL of homogenization buffer (20 mM HEPES, pH 7, 0.1 M KCl, protease inhibitors)

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