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, GRX1-roGFP2 or TRaQ-G) enable real-time, organelle-specific redox readouts but primarily report redox potential rather than absolute GSH levels, whereas fractionation coupled with mass spectrometry provides absolute GSH/GSSG quantification but remains static and susceptible to lysis-associated artifacts

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After 12 h, the medium was replaced by normal growth medium containing 100 g/mL of cycloheximide (CHX, #C7698, Sigma-Aldrich, Shanghai, China)

Eakin GS, Peterson S

The suppression of C/EBP entirely counteracted the transcriptional regulation of Ybx1 over PPAR, affirming the critical role of a Ybx1/C/EBP complex in DIO hepatocytes