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Primers for Bmal1, Clock, Per 1-3, Cry1-2 and -actin were synthesised by Integrated DNA Technologies (IDT, Iowa, USA)

Reagents A 30% hydrogen peroxide solution (H1009) with nicotinamide (N0636) was purchased from SigmaAldrich (St

Peptide pH, salt form, purity, residual solvents, storage history, and lyophilisation quality can all affect reconstitution

Fig.1 Affinity chromatography involves three major steps: loading, separation, and elution With the most advanced AC technique and years of experience in the field of purification, Creative Biostructure now can provide custom affinity chromatography service

Glutathione, a powerful antioxidant naturally produced by the body, has long been revered for its ability to combat oxidative stress and inflammation

Cystine in SLC7A11-overexpressing cells cannot be reduced to cysteine, resulting in increased levels of disulfide and disulfidptosis (Liu et al., 2023a)