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Low calorie dieting increases cortisol

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Following acclimation for seven days, mice were single-housed and maintained on HFD for 4 additional weeks

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The Epworth Sleepiness Scale (ESS), Athens Insomnia Scale (AIS), and Pittsburg Sleep Quality Index (PSQI) were used to quantify excessive daytime sleepiness (EDS), insomnia symptoms, and sleep quality, respectively