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The 25-l reaction solution contained 6 g of nuclear extract , 5 l of 5 binding buffer (50 mM Tris-HCl, pH 7.4, with 20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 5 mM DTT, and 0.25 mg/ml poly dI-dC) and 50,000 cpm labeled probe

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10.4236/as.2014.513133 94 HusseinN

Regulatory CD4+CD25+ T cells dampen inflammatory disease in murine Mycoplasma pneumonia and promote IL-17 and IFN- responses

JCR: J Clin Rheumatol (2014) 20:14650

This difference reflects common commercial testing practices rather than the fact that the compound cannot be analysed chromatographically