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A study has shown that organic acids may exert bacterial inhibitory effects by disrupting the bacterial internal balance and inhibiting enzyme activity (101)

The use of relatively more infrequent restriction sites as a selection marker include - in a non-limiting fashion - preferably those sites composed at least a 4 nucleotide sequence, more preferably those composed at least a 5 nucleotide sequence, or those composed at least a 6 nucleotide sequence (e.g

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