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Several checkpoints were performed in order to assess parasite fitness and tail-loss during transformation

The authors also found that FLNA is required for SSTR2 mediated inhibition of cell survival, and the molecular mechanism involves a competition of FLNA with p85, the regulatory subunit of PI3K, for direct binding to SSTR2

The result should ideally be "ND" or within safe limits

In the second scenario, the final yield of your elution is close to 100 mg of GST-fusion protein, so youre feeling confident that you used the right binding buffer and that your protein isnt too big

These compositional shifts undermine membrane fluidity and integrity, thereby impairing neuronal function