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Disrupting this interaction by substituting E134 and D137 in BubR1 with positively charged residues individually or combinatorially had no effect on kinetochore localization, but almost completely abolished SAC signaling, a phenotype similarly observed when replacing Cdc20 R286 with a negatively charged residue (Supplementary Fig

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In this section, we summarize how metabolic interventions sensitize cancer cells to these therapies, offering a potential avenue for combination therapy