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doi:10.1098/rsif.2023.0050

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Next, coverslips were washed three times with 2 SSC and treated with pre-hybridization solution (30% formamide (Thermo Fisher Scientific, AM9344) and 2 SSC) and incubated for at least three hours at 37 C

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The process of increasing blood flow to exercising musculature involves shunting of blood from non-active tissues to working muscle