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The standard reaction buffer for the Adapta TR-FRET assay contained 10mM Tris-HCI pH 7.5, 3mM MgCI 2 , 50mM NaCI, 1 mM DTT, 0.05% CHAPS ((3-[(3- cholamidopropyl)dimethylammonio]-1-propanesulfonate)

This is one of the things that makes it different from community-derived peptide protocols

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Subsequently, the cells were washed with PBS and stained by ROS Green TM H 2 O 2 Probe

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