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For MTX, the HPLC retention time, which was determined to be 0.96 0.02 min, showed good method reproducibility (Fig

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The liberated CoA-SH reacts with 5,5-dithiobis-(2-nitrobenzoic acid) and forming thiophenolate ion, whose generation is proportional to the amount of carnitine and can be measured spectrophotometrically at 412 nm

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