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( C F ) Luciferase activity (fold change) in AC16 cells transfected with reporter plasmids containing the following human PPAR coactivator 1- ( PPARGC1A ) promoter fragments: pGL3-basic vector(BV)- PPARGC1A -1631 ( C ), pGL3BV-a- PPARGC1A -1386 ( D ), pGL3BV-a- PPARGC1A -1012 ( E ), pGL3BV-a- PPARGC1A -210 ( F ), followed by treatment with 50 M rosiglitazone, 50 M WY-14643, or a combination of both ( n = 412)