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To further substantiate these results, we measured the expression of hexokinase 1 (HK1), a rate limiting enzyme of glycolysis

At the center of this defense is glutathione, a molecule often called the "master antioxidant." While your body produces it naturally, factors like aging, poor sleep, and environmental load can deplete your levels over time

2012;4:127ra37

Supernatants were diluted with ChIP dilution buffer (50 mM Tris-HCl [pH 8.0], 167 mM NaCl, 1.1% Triton X-100, 0.11% NaDoc, 200 nM PMSF), precleared with normal rabbit IgG (Cell Signaling Technology, #2729) or normal mouse IgG (Santa Cruz, sc-2025), and incubated with Protein G agarose beads (Thermo Scientific, 20398) pre-washed in 3.125 mg/mL BSA/ChIP dilution buffer for 2 h at 4 C

Furthermore, transgenic plants expressing the redox-sensitive fluorescent biomarker roGFP2 indicated more oxidative conditions during AITC treatment

Cell Biol Int (2017) 41:61121