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PMID: 27847094

Together with previous work showing that perturbations of the N-terminal loop or -SNAP/Sec17 membrane engagement compromise SNARE complex disassembly and membrane fusion dynamics 11,37,38 , these findings support the idea that the M105I mutation may impair membrane fusion not only by diminishing plasma membrane binding, but also by diverting -SNAP into non-productive, off-pathway membrane contacts that cannot support a properly assembled 20S complex

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Research has indicated that dietary choices, particularly high-fat diets, significantly influence the gut microbiota, exacerbating DED symptoms through mechanisms such as decreased tear secretion, increased oxidative stress, and heightened apoptosis (Wu et al., 2020)

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Question: Reconstitute half vial now, add more water to other half later