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Ferroptosis is also associated with excess Fe3+ in the intracellular medium

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To exclude non-specific hits, we further validated these hits in TNF/cycloheximide (CHX)-induced HepG2 liver cell pyroptosis model, which was mediated by caspase-3-GSDME pathway 20

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We analysed the impact of NOx application after 48 h of the treatment, while the embryos were cultured on distilled water, under optimal conditions for germination

To test microtubule (MT) requirements for juxtanuclear IFD concentration, we treated P mec-7 GFP::IFD-1 expressing animals with the potent microtubule inhibitor colchicine 29 from L4 to Ad2 (Fig