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After integrating these datasets, the overall PBMC landscape remained stable: 11 major immune cell groups were identified using similar clustering structure and canonical marker gene expression (Supplementary Fig

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This process, often referred to as target validation , is a foundational step in early drug development

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When NNMT activity is elevated as documented in obese adipose tissue, several cancer types, and aged skeletal muscle intracellular NAD+ availability decreases, SAM availability decreases, and downstream sirtuin activity (particularly SIRT1 and SIRT3) is reduced