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Chapman and Hall/CRC, pp 1035 Zhang Z et al (2016) Autophagy in neurodegenerative diseases and metal neurotoxicity

At mentioned time points, MTT solution was added, incubated in darkness for 4 h at 37 C, and then, replaced by dimethyl sulfoxide to dissolve the formazan crystals and finally absorbance was read at 570 nm by a plate reader