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For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min

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Brain 135 , 30743088 (2012)

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Some problems of the gut can also be a cause of vitamin B12 deficiency, but this is less uncommon, for example: surgery to remove parts of the bowel (the stomach or the end of the small intestine) Crohn's disease atrophic gastritis (where the lining of the stomach is thinned)