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While the IOM had based B12 needs on the amount required to correct hematological parameters for people with pernicious anemia, the EFSA had almost 20 more years of research to draw on, and concluded that the most suitable approach was basing needs on biomarkers (serum B12, holotranscobalamin, MMA, and homocysteine)

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As reported for isatuximab and daratumumab, mass spectrometry analyses and the use of specific IFE-reflex assays (i.e., isatuximab Hydrashift assay, DIRA assay) can overcome interference by these therapeutic antibodies and allow more accurate measurements of serum M-protein in MM patients [48, 53,54,55]