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Xiao L, et al

PMID: 18469287

Nilsson-rtman, V., Stoks, R., De Block, M., Johansson, H

A.BuehnerG.ChangY.HarperJ

The WT and KO cells at day 8 of spontaneous differentiation were dispersed to single cells using TrypLE Select (Thermo Fisher Scientific, Netherlands), counted, spun down at 500 x g for 5 min at 4 C and resuspended at a concentration of 5 104 cells/50 L per biological replicate in cold ATAC-RSB (10 mM Tris-HCl (Bio-Shop, Canada) pH = 7.4, 3 mM MgCl 2 (Millipore, Germany), 10 mM NaCl (Bio-Shop, Canada), containing 0.1% (v/v) Tween-20 (Roche Diagnostics, Germany), 0.1% (v/v) NP-40 (Biosciences, USA))