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After blocking with 5% BSA diluted in Tris-buffered saline with 0.1% Tween 20 (TBST) for 1 h at 37C, the membranes were incubated overnight at 4C with primary antibodies against LC3B, cleaved-Caspase 3 (Abcam

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Midbrain organoids with an SNCA gene triplication model key features of synucleinopathy