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Because water is amphiprotic, one water molecule can react with another to form an OH ion and an H 3 O + ion in an autoionization process: Equation 16.4 The equilibrium constant K for this reaction can be written as follows: Equation 16.5 When pure liquid water is in equilibrium with hydronium and hydroxide ions at 25C, the concentrations of the hydronium ion and the hydroxide ion are equal: [H 3 O + ] = [OH ] = 1.003 10 7 M

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Students t-tests were performed for statistical analysis and p-values of less than 0.05 with two-tailed analysis were considered statistically significant

Edgcomb, S

For the assessment of membrane integrity (Extended Data Fig