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Subsequent quantitative reverse transcriptase PCRs were performed using the StepOne Plus RT-PCR system (Applied Biosystems, Waltham, MA, USA) with SYBR Premix Ex Taq II (Takara Bio, Kusatsu, Japan) and primers for amplifying human ALP , VEGFA , and the reference gene GAPDH (see Table 1)

We used the SCP1039, SCP1288, SCP1493, SCP398, and SCP1162 data sets from the Broad Institute Single Cell Portal

Intriguingly, the authors hypothesize progesterone amplification of this pathway through PGRMC1, noting E6s selective degradation of nuclear progesterone receptors (sparing membrane isoforms), which may sustain autophagy-mediated immune evasion in HPV oncogenesis [183, 184]

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As strength of aggregation increases, RBCs form rouleaux and then clumps