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Your lens is now off your camera Depth of Field Button On certain DSLR such as the Canon 7D there is a depth of field preview button, pressing this shows you the best depth of field for your chosen settings

Prior to transfer, stain-free gels were imaged on a Biorad Chemidoc MP Imaging System (Biorad, Hercules, CA) to confirm equal protein load, and were then wet transferred onto PVDF membranes at 100 V constant for 1 h and equal transfer was routinely confirmed by Ponceau S stain

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Results: Of the 296 patients, 190 (64.2%) were female, and the mean age was 61 years

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