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Activation of TGFB/SMAD signaling pathway in HOXC8-imESCs In the TGFB signaling pathway, SMAD2 and SMAD3 are activated via phosphorylation by TGFB receptors and translocated into the nucleus to regulate gene expression 29,30

For the genomes of the evolved strains sequenced by MiSeq sequencing only, the resulting read data (FASTQ files) were processed with the CLC Genomics Workbench software (Qiagen Aarhus A/S) for base quality filtering and read trimming

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