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Cannabinoid type 2 receptor activation inhibits MPP+-induced M1 differentiation of microglia through activating PI3K/Akt/Nrf2 signal pathway

A major disadvantage for using GST as solubility and affinity tag relies on its oligomerized form: GST has four solvent exposed cysteines that can provide a significant oxidative aggregation (Kaplan et al., 1997), making it a poor choice for tagging oligomeric target proteins (Malhotra, 2009)

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The functional relevance of these interactions warrants further investigation

The range of detectable binding affinities depends on the compound concentration used in the experiment and the affinity of each RNA-small molecule pair